Tec-Control Chromatography

 

For radiopharmaceutical quality control

Tec-Control Chromatography tests the radiochemical purity of specific Tc-99m-labeled radiopharmaceuticals. The accompanying chart shows which strips and solvents are
required to perform each individual test. Some solvents must be purchased separately
(see Sigma-Aldrich chart) due to hazardous material shipping restrictions.

Detailed instruction manuals are packaged with each strip container, although our
Radiopharmaceutical QC Procedure Manual (151-000) explains paper chromatography
in greater detail.

TEC-CONTROL ALUMINUM BREAKTHRU KIT

Simple 3-step procedure

The Aluminum Breakthru Kit provides a rapid, easy and inexpensive way to test aqueous
solutions, particularly pertechnetate generator eluate, for trace quantities of aluminum.
Aluminum forms an intense red precipitate with the indicator paper, and the intensity of the
color is directly proportional to the amount of aluminum in the solution. The USP allows a
concentration of aluminum ion in an injection ≤10 micrograms per milliliter (10 µg/ml) in
technetium 99m eluate prepared from Molybdenum 99 formed as a result of uranium fission.

Simple Procedure:

1.	Place a drop of the eluate or solution to be tested on the indicator paper. The best procedure is to form a hanging drop using a 19-22G needle.
2.	Place a drop of the standard aluminum solution on the indicator paper. Use the same size drop.
3.	Compare the intensity of the red spot formed. If the eluate spot is less intense than the standard solution, the eluate contains less than 10µg/ml aluminum.

 

150-780 Chromatography Kit, Tec-Control Aluminum Breakthru Kit includes: Aluminum standard, 5 ml, 10 ug/ml, 50 indicator strips and manual

151-000 Procedure Manual, Radiopharmaceutical QC

References:

Miniaturized Chromatographic Quality-Control Procedures for Tc-99m Radiopharmaceuticals;
A. Michael Zimmer and Dan G. Pavel, Journal of Nuclear Medicine, Vol. 18/12, Dec. 1977, pg. 1230.
Technical Parameters Associated with Miniaturized Chromatography Systems; Raimund A. Taukulis,
A. Michael Zimmer, Dan G. Pavel and Bhupendra A. Patel, University of Illinois Medical Center,
Chicago, Illinois, Journal of Nuclear Medicine Technology, Vol. 7/1.

RADIOPHARMACEUTICAL SOLVENT(S) REQUIRED* STRIPS REQUIRED Aluminum Breakthru 150-781 150-782 Bicisate (Neurolite™) Ethyl Acetate 99.% 150-130 Diphosphonate Acetone (HPLC Grade), Distilled H2O 150-001 & 150-005 Disofenin (Hepatolite™) 150-160, Distilled H2O 150-122 & 150-125 DMSA Acetone (HPLC Grade), 150-025 DTPA Acetone (HPLC Grade), Distilled H2O 150-001 & 150-005 Exametazine (Ceretec™) Ethyl Acetate 99.% 150-130 Glucoheptonate Acetone (HPLC Grade), Distilled H2O 150-001 & 150-005 HDP Acetone (HPLC Grade), Distilled H2O 150-001 & 150-005 MAA Acetone (HPLC Grade), 150-001 MAG3 (Mertiatide™) Acetone, (HPLC Grade), Chloroform 99.8% Tetrahydrofuran 99+% 150-951 & 150-952 MDP Acetone (HPLC Grade), Distilled H2O 150-001 & 150-005 Mebrofenin (Cholotec™) 150-160, Distilled H2O 150-122 & 150-125 Pyrophosphate Acetone (HPLC Grade), Distilled H2O 150-001 & 150-005 Sestamibi (Cardiolite™ and Miraluma™ Ethyl Acetate 99.% 150-991 Sulphur Colloid Acetone (HPLC Grade) 150-001 Tc-99m (reduced) Acetone (HPLC Grade), 150-001 Tc-99m Monocolonal Antibodies 0.9% Saline 150-771 Tetrofosmin (Myoview™) Ethyl Acetate 99.% 150-971 In-111, Octreotide (Octreoscan™) 150-773 & 0.9% Saline 150-771 ** In-11 Monocolonal Antibodies 150-773 & 0.9% Saline 150-771 ** In-111/Y-90 Zevalin™ 150-773 & 0.9% Saline 150-771 I-131 Monoclonal Antibodies And Neutrospec™ 0.9% Saline 150-771

* See Sigma-Aldrich product matrix for solvents.
** Note: A Well Plate is suggested to perform QC on these radiopharmacuticals
	Contact Fisher Scientific Corp. 800-766-7000 / www.fishersci.com / Item #14-245-71

Tec-Control Solvent Vendor: Sigma-Aldrich Chemical Company 800-558-9160 / www.sigmaaldrich.com Note: Customers outside the US should visit Sigma-Aldrich web site to locate a regional office.
Solvent Description	Vendor Part #
Acetone HPLC Grade	27072-5
Ethyl Acetate 99.5% ACS Reagent	31990-2
Chloroform 99.8% ACS Reagent	31998-8
Tetrahydrofuran 99+% ACS Reagent	36058-9

Chromatography Strips 150-001 Strips, RED, 50/pkg   150-005 Strips, BLACK, 50/pkg   150-025 Strips, YELLOW, 50/pkg   150-122 Strips, ORANGE, 50/pkg   150-125 Strips, LIGHT BLUE, 50/pkg   150-130 Strips, GOLD, 50/pkg   150-771 Strips, DARK GREEN, 50/pkg   150-951 Strips, LIME, 50/pkg   150-952 Strips, PEACH, 50/pkg   150-971 Strips, TEAL, 50/pkg   150-991 Strips, PINK, 50/pkg   150-782 Strips, ALUMINUM, 50/pkg   Chromatography Solvents 150-160 Solvent, 20% Sodium Chloride, 30 ml   150-773 Solvent, DTPA, 5 ml   150-781 Solvent, Aluminum Standard, 5 ml, 10 µg/ml   Related Items: 150-960 Developing Vials, 10 ml, 288/case (used for all Tec-Control testing)   150-961 Developing Vials, 5 ml, 144/case (used for Sestamibi & Tetrofosmin)   066-533 Forceps, Curved, Locking, 9.5" l (24.1 cm)   066-535 Forceps, Straight, Locking, 9.5" l (24.1 cm)   066-536 Forceps, Curved, Non-Locking, 13.5" l (34.3 cm)

Radiopharmaceutical

QC Manual

This 90-page manual explains Paper Chromatography, a QC method for evaluating the radiochemical purity of currently used Tc-99m-labeled radiopharmaceuticals. Procedures are quick and easy to use, a simple quality control solution for any nuclear medicine department.